The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) technology is a validated in vitro model, designed to simulate the microbial ecology of the gastrointestinal tract from stomach to distal colon. This instrument could allow to get more insight on the direct effects elicited by dietary advanced end products, postbiotics, probiotics on human gut microbiome composition and function. The procedure includes: inoculation of a fecal samples, addition of different compunds that you want to test, fermentation and collection of samples for gut microbiome and metabolomics analyses.